This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CR1 / CD35 Antibody (clone E11, Preservative Free) LS-B2556
catalog :
LS-B2556
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E11

The same clone is also sold as:
reactivity :
human
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
85031
AntibodyName :
LS-B2556
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CR1 / CD35 Antibody (clone E11, Preservative Free) LS-B2556
Specificity :
Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
ClonalityDesc :
Monoclonal
CloneName :
E11
AntibodyModification :
Preservative Free Unconjugated
PresentationDesc :
PBS
ImmunogenType :
Cells
ImmunogenDesc :
Human acute monocytic leukaemia cells.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
CR1 / CD35
StandardGeneSymbol :
CR1
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Human
Usage :
Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 µg/ml)
ShortWebDescription :
CD35 antibody LS-B2556 is an unconjugated mouse monoclonal antibody to human CD35 (CR1). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.
Synonyms :
CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.