product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SFTPA1 / Surfactant Protein A Antibody LS-B2543
catalog :
LS-B2543
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Jin H, Zangar R. High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Curr Protoc Toxicol. 2012;Chapter 17:Unit 17.15 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2543 image 1
Anti-SFTPA1 / Surfactant Protein A antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
85018
AntibodyName :
LS-B2543
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SFTPA1 / Surfactant Protein A Antibody LS-B2543
Specificity :
Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. In immunohistochemical staining from paraffin embedded tissue samples, this antibody reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4, 15 mM Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Native SP-A purified from bronchioalveolar lavage from proteinosis patients.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
SFTPA1 / Surfactant Protein A
StandardGeneSymbol :
SFTPA1
Reactivity :
Human
Usage :
ELISA (1:8000), IHC, IHC-P (10 µg/ml), WB (1:500)
ShortWebDescription :
Surfactant Protein A antibody LS-B2543 is an unconjugated mouse monoclonal antibody to human Surfactant Protein A (SFTPA1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2543 was determined to be 10 ug/ml.
Synonyms :
SFTPA1, Alveolar proteinosis protein, COLEC4, Collectin-4, PSP-A, PSPA, SFTP1, SFTPA, SFTPA1B, SPA1, SP-A, SP-A1, SPA, Surfactant Protein A, Surfactant protein A1, Surfactant protein A1B, Surfactant A
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.