This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ AKT1S1 / PRAS40 Antibody LS-B2541
catalog :
LS-B2541
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
85016
AntibodyName :
LS-B2541
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ AKT1S1 / PRAS40 Antibody LS-B2541
Specificity :
Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.2, 0.09% Sodium Azide, 1% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant full length human PRAS40 expressed in E. coli.
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
AKT1S1 / PRAS40
StandardGeneSymbol :
AKT1S1
Reactivity :
Human
Usage :
IHC, IHC-P (10 µg/ml), IP, WB (0.1 - 1 µg/ml)
ShortWebDescription :
PRAS40 antibody LS-B2541 is an unconjugated mouse monoclonal antibody to human PRAS40 (AKT1S1). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.
Synonyms :
AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.