product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) LS-B2540
catalog :
LS-B2540
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7C5G1H6H10
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Reference
Ulloa L, Tabibzadeh S. Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor. J Biol Chem. 2001;276:21397-404 pubmed
Tsukui T, Capdevila J, Tamura K, Ruiz Lozano P, Rodriguez Esteban C, Yonei Tamura S, et al. Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1. Proc Natl Acad Sci U S A. 1999;96:11376-81 pubmed
image
image 1 :
LifeSpan Biosciences LS-B2540 image 1
Anti-LEFTY2 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
85015
AntibodyName :
LS-B2540
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) LS-B2540
Specificity :
Reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
7C5G1H6H10
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a,k
Gene :
TGFB4 / LEFTY2
StandardGeneSymbol :
LEFTY2
gene family :
TGF beta
Reactivity :
Human
Usage :
ELISA (1:5000), IHC, IHC-P (10 µg/ml), WB (1:2000)
ShortWebDescription :
LEFTY2 antibody LS-B2540 is an unconjugated mouse monoclonal antibody to human LEFTY2 (TGFB4). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B2540 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2540 was determined to be 10 ug/ml.
Synonyms :
LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.