This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CHEMR23 / CMKLR1 Antibody (N-Terminus, clone BZ332) LS-B2537
catalog :
LS-B2537
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
BZ332
reactivity :
human
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
85012
AntibodyName :
LS-B2537
TargetSpecies :
Human
Host Species :
Rat
Product Name :
IHC-plus™ CHEMR23 / CMKLR1 Antibody (N-Terminus, clone BZ332) LS-B2537
Specificity :
Recognizes human serpentine chemokine-like receptor 1 (CMKLR1), a novel chemoattractant receptor, which is also referred to Chem23 or DEZ. CMKLR1 is expressed on plasmacytoid dendritic cells (pDC) in blood but is not expressed on myeloid dendritic cells, lymphocytes, monocytes or granulocytes. Studies show that CMKLR1 is also expressed on dendritic cells, which have been generated in vitro from monocytes. The sole ligand for CMKLR1 is chemerin, a chemoattractant that exists at the mRNA level in a wide range of tissues, and as an inactive proform in serum and plasma. Studies suggest that chemerin, in plasma, is converted into a potent pDC attractant following blood coagulation and may be a process by which pDC's are recruited to sites of bleeding and tissue damage.
ClonalityDesc :
Monoclonal
CloneName :
BZ332
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Peptide corresponding to an epitope within the N-Terminus of human CMKLR1
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG2a
Gene :
CHEMR23 / CMKLR1
StandardGeneSymbol :
CMKLR1
gene family :
GPCR
Subfamily :
Chemoattractant
Reactivity :
Human
Usage :
Flo (1:25 - 1:100), IHC, IHC-P (10 µg/ml)
ShortWebDescription :
CMKLR1 antibody LS-B2537 is an unconjugated rat monoclonal antibody to human CMKLR1 (CHEMR23) (N-Terminus). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2537 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2537 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
Synonyms :
CMKLR1, Chemokine receptor-like 1, DEZ, CHEMERINR, Resolvin E1 receptor, RVER1, Chemokine-like receptor 1, ChemR23, G-protein coupled receptor DEZ
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.