This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ FAK / Focal Adhesion Kinase Antibody (phospho-Tyr576) LS-B2522
catalog :
LS-B2522
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
84997
AntibodyName :
LS-B2522
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ FAK / Focal Adhesion Kinase Antibody (phospho-Tyr576) LS-B2522
Specificity :
Reactivity: Human FAK. Mouse, rat, chicken and frog (100% homologous) FAK have not been tested, but are expected to react. Some cross-reactivity with Pyk2 may still be experienced in cases where Pyk2 is overexpressed relative to FAK within the same cell system.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pTyr576
PresentationDesc :
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic phospho-peptide
ImmunogenDesc :
Synthetic phosphopeptide derived from the region of human FAK that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
FAK / Focal Adhesion Kinase
StandardGeneSymbol :
PTK2
gene family :
Protein Kinase
Subfamily :
Focal Adhesion Kinase
Reactivity :
Frog, Chicken, Mouse, Rat, Human
Usage :
ELISA, IHC, IHC-P (1:100), WB
ShortWebDescription :
Focal Adhesion Kinase antibody LS-B2522 is an unconjugated rabbit polyclonal antibody to Focal Adhesion Kinase (FAK) (pTyr576) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2522 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2522 was determined to be 1:100. Positive control: Chicken embryo fibroblasts (CEFs) expressing FAK protein and plated on fibronectin and treated with vanadate.
Synonyms :
PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.