IHC-plus™ SKP2 Antibody LS-B2487 | LifeSpan Biosciences LS-B2487 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ SKP2 Antibody LS-B2487

catalog :

LS-B2487

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:50; fig 3	Parameswaran B, Chiang H, Lu Y, Coates J, Deng C, Baer R, et al. Damage-induced BRCA1 phosphorylation by Chk2 contributes to the timing of end resection. Cell Cycle. 2015;14:437-48 pubmed publisher

image

image 1 :

Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

84534

AntibodyName :

LS-B2487

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ SKP2 Antibody LS-B2487

Specificity :

Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

PresentationDesc :

PBS, pH 7.4, 0.09% Sodium Azide

ImmunogenType :

Recombinant protein

ImmunogenDesc :

Recombinant, full-length human p45SKP2 protein.

PurificationDesc :

Protein A purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG1,k

Gene :

SKP2

StandardGeneSymbol :

SKP2

Reactivity :

Human

Usage :

ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB (1 - 2 µg/ml)

ShortWebDescription :

SKP2 antibody LS-B2487 is an unconjugated mouse monoclonal antibody to human SKP2. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B2487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2487 was determined to be 5 ug/ml. Positive control: HeLa, Jurkat and 293T cell lines.

Synonyms :

SKP2, F-box/LRR-repeat protein 1, FBL1, FLB1, F-box protein Skp2, FBXL1, p45, p45skp2

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.