antibody

IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2486

LS-B2486

polyclonal

domestic rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-B2486

Rat

Rabbit

IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2486

Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

Polyclonal

Unconjugated

PBS, 0.05% Sodium Azide

Recombinant protein

E. coli expressed active rat 92 kDa type IV collagenase (catalytic domain)

Protein A purified

Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG

MMP9 / Gelatinase B

MMP9

Protease

Metallopeptidase M10A

Mouse, Rat, Human

IHC, IHC-Fr (1:50), IHC-P (5 µg/ml), IP (1:250), WB (1:1000)

Gelatinase B antibody LS-B2486 is an unconjugated rabbit polyclonal antibody to Gelatinase B (MMP9) from rat. It is reactive with human, mouse and rat. Validated for IHC, IP and WB. Cited in 4 publications.

Immunohistochemistry: LS-B2486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2486 was determined to be 5 ug/ml. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

MMP9, 92kd gelatinase, 92 kDa gelatinase, Matrix metalloproteinase-9, MMP-9, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2

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