PathPlus™ SERPINF1 / PEDF Antibody (aa196-347) LS-B2471 | LifeSpan Biosciences LS-B2471 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

PathPlus™ SERPINF1 / PEDF Antibody (aa196-347) LS-B2471

catalog :

LS-B2471

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Rowland T, Blaschke A, Buchholz D, Hikita S, Johnson L, Clegg D. Differentiation of human pluripotent stem cells to retinal pigmented epithelium in defined conditions using purified extracellular matrix proteins. J Tissue Eng Regen Med. 2013;7:642-53 pubmed publisher

image

image 1 :

Anti-PEDF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

84518

AntibodyName :

LS-B2471

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

PathPlus™ SERPINF1 / PEDF Antibody (aa196-347) LS-B2471

Specificity :

Recognizes human GST-PEDF fusion protein with a MW of ~46kD.

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa196-347

PresentationDesc :

PBS, pH 7.6, 0.09% Sodium Azide

ImmunogenType :

Recombinant protein

ImmunogenDesc :

Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-Terminus was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.

PurificationDesc :

Protein A purified

RecommendedStorageDesc :

Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG2a

Gene :

SERPINF1 / PEDF

StandardGeneSymbol :

SERPINF1

Reactivity :

Human

Usage :

ELISA, ICC, IHC, IHC-Fr, IHC-P (5 µg/ml), WB

ShortWebDescription :

PEDF antibody LS-B2471 is an unconjugated mouse monoclonal antibody to human PEDF (SERPINF1) (aa196-347). Validated for ELISA, ICC, IHC and WB. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B2471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2471 was determined to be 5 ug/ml.

Synonyms :

SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.