This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PKM / Pyruvate Kinase, Muscle Antibody LS-B2445
catalog :
LS-B2445
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
83788
AntibodyName :
LS-B2445
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ PKM / Pyruvate Kinase, Muscle Antibody LS-B2445
Specificity :
Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
PKM / Pyruvate Kinase, Muscle
StandardGeneSymbol :
PKM
Reactivity :
Mouse, Rat, Human, Monkey
Usage :
IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:1000)
ShortWebDescription :
Pyruvate Kinase, Muscle antibody LS-B2445 is an unconjugated rabbit polyclonal antibody to Pyruvate Kinase, Muscle (PKM) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2445 was determined to be 1:100.
Synonyms :
PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.