product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MUC5AC Antibody (clone CLH2) LS-B2434
catalog :
LS-B2434
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CLH2
reactivity :
human
application :
ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Issa S, Schulz B, Packer N, Karlsson N. Analysis of mucosal mucins separated by SDS-urea agarose polyacrylamide composite gel electrophoresis. Electrophoresis. 2011;32:3554-63 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2434 image 1
Anti-MUC5AC antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
83777
AntibodyName :
LS-B2434
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ MUC5AC Antibody (clone CLH2) LS-B2434
Specificity :
Recognizes the MUC5AC tandem repeat of human mucin. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa.
ClonalityDesc :
Monoclonal
CloneName :
CLH2
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.6, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide of the MUC5AC tandem repeat.
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
MUC5AC
StandardGeneSymbol :
MUC5AC
Reactivity :
Human
Usage :
ELISA, ICC, IHC, IHC-Fr (1:500 - 1:1000), IHC-P (10 µg/ml), IP
ShortWebDescription :
MUC5AC antibody LS-B2434 is an unconjugated mouse monoclonal antibody to human MUC5AC. Validated for ELISA, ICC, IHC and IP. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2434 was determined to be 10 ug/ml. Positive control: Normal human epithelial tissues: bronchus, endocervix, stomach and gallbladder.
Synonyms :
MUC5AC, Gastric mucin, Major airway glycoprotein, Muc5/5ac, Mucin-5AC, LeB, Lewis B blood group antigen, Tracheobronchial mucin, TBM, MUC-5AC, MUC5
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.