This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ORAI3 Antibody (N-Terminus) LS-B2385
catalog :
LS-B2385
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
83632
AntibodyName :
LS-B2385
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ORAI3 Antibody (N-Terminus) LS-B2385
Specificity :
Recognizes human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. AHP1496 is not expected to cross react with either ORAI1 or ORAI2.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A 15 AA peptide near the N-Terminus of human ORAI3
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG
Gene :
ORAI3
StandardGeneSymbol :
ORAI3
Reactivity :
Human
Usage :
IHC, IHC-P (5 µg/ml), WB
ShortWebDescription :
ORAI3 antibody LS-B2385 is an unconjugated rabbit polyclonal antibody to human ORAI3 (N-Terminus). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2385 was determined to be 5 ug/ml. Western Blot: LS-B2385 detects a band of approximately 35kD in A20 cell lysates.
Synonyms :
ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.