product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SLC12A4 / KCC1 Antibody (aa997-1007) LS-B2377
catalog :
LS-B2377
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Sunuwar L, Asraf H, Donowitz M, Sekler I, Hershfinkel M. The Zn2+-sensing receptor, ZnR/GPR39, upregulates colonocytic Cl- absorption, via basolateral KCC1, and reduces fluid loss. Biochim Biophys Acta Mol Basis Dis. 2017;1863:947-960 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2377 image 1
Antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B2377 image 2
Anti-SLC12A4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
83624
AntibodyName :
LS-B2377
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ SLC12A4 / KCC1 Antibody (aa997-1007) LS-B2377
Specificity :
Human SLC12A4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa997-1007
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-DPSHAPDNFRE, from the internal region of the protein sequence according to NP_005063.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
SLC12A4 / KCC1
BlockingPeptide :
LS-E26489
StandardGeneSymbol :
SLC12A4
gene family :
Transporter
Subfamily :
Cation:chloride symporter
Reactivity :
Mouse, Dog, Rat, Hamster, Bat, Human
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:4000), WB (0.3 - 1 µg/ml)
ShortWebDescription :
KCC1 antibody LS-B2377 is an unconjugated goat polyclonal antibody to KCC1 (SLC12A4) (aa997-1007) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2377 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2377 was determined to be 5 ug/ml.
Synonyms :
SLC12A4, Erythroid K-Cl cotransporter 1, HKCC1, KCC1, K-Cl cotransporter, Erythroid K:Cl cotransporter
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.