This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CTNNB1 / Beta Catenin Antibody (C-Terminus) LS-B2354
catalog :
LS-B2354
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
83542
AntibodyName :
LS-B2354
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ CTNNB1 / Beta Catenin Antibody (C-Terminus) LS-B2354
Specificity :
Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.4, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A 15 AA synthetic peptide derived from the C-Terminus of human and mouse b-catenin protein.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
CTNNB1 / Beta Catenin
StandardGeneSymbol :
CTNNB1
gene family :
Catenin
Reactivity :
Xenopus, Chicken, Mouse, Rat, Human
Usage :
ELISA (0.1 - 1 µg/ml), IHC, IHC-P (2.5 µg/ml), IP, WB
ShortWebDescription :
Beta Catenin antibody LS-B2354 is an unconjugated rabbit polyclonal antibody to Beta Catenin (CTNNB1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2354 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2354 was determined to be 2.5 ug/ml. Western Blot after IP: 1-2 ug/ml. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 ug. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.
Synonyms :
CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.