This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-BCL2 / Bcl-2 Antibody (aa41-54, Azide-free) IHC-plus™ LS-B2352
catalog :
LS-B2352
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
83540
AntibodyName :
LS-B2352
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
Anti-BCL2 / Bcl-2 Antibody (aa41-54, Azide-free) IHC-plus™ LS-B2352
Specificity :
Recognizes human Bcl-2a at ~25kD. Species cross-reactivity: monkey. Does not react with mouse or rat.
ClonalityDesc :
Monoclonal
AntibodyModification :
Azide-free Unconjugated
AntigenModification :
aa41-54
PresentationDesc :
PBS, pH 7.4.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to residues 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein. Cellular Localization: Cytoplasm. MW of antigen: 25-26kD. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%); Bovine (93%); Horse (86%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
+4°C or -20°C, Avoid repeated freezing and thawing.
IsotypeName :
IgG1
Gene :
BCL2 / Bcl-2
StandardGeneSymbol :
BCL2
gene family :
Apoptosis
Reactivity :
Dog, Pig, Human, Monkey
Usage :
ELISA, Flo, IF, IHC-P (5 µg/ml), WB (1 µg/ml)
UsageText :
Immunohistochemistry: LS-B2352 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2352 was determined to be 5 ug/ml. Western Blot: 1 ug/ml for 2hrs at RT. Positive control: Tonsil or follicular lymphomas. Raji or HeLa cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Synonyms :
BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.