product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11) LS-B2321
catalog :
LS-B2321
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SAM11
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; loading ...; fig 1a
  • immunohistochemistry; mouse
  • western blot; mouse
Nasri I, Bonnet D, Zwarycz B, d Aldebert E, Khou S, Mezghani Jarraya R, et al. PAR2-dependent activation of GSK3? regulates the survival of colon stem/progenitor cells. Am J Physiol Gastrointest Liver Physiol. 2016;311:G221-36 pubmed publisher
Rolland Fourcade C, Denadai Souza A, Cirillo C, Lopez C, Jaramillo J, Desormeaux C, et al. Epithelial expression and function of trypsin-3 in irritable bowel syndrome. Gut. 2017;66:1767-1778 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2321 image 1
Anti-F2RL1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
82909
AntibodyName :
LS-B2321
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11) LS-B2321
Specificity :
Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
ClonalityDesc :
Monoclonal
CloneName :
SAM11
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to the N-Terminus of human PAR2 receptor.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
F2RL1 / PAR2
StandardGeneSymbol :
F2RL1
gene family :
GPCR
Subfamily :
Proteinase-activated
Reactivity :
Human
Usage :
ELISA (0.1 - 1 µg/ml), IF (1 - 3 µg/ml), IHC, IHC-P (5 µg/ml)
ShortWebDescription :
PAR2 antibody LS-B2321 is an unconjugated mouse monoclonal antibody to human PAR2 (F2RL1) (N-Terminus). Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2321 was determined to be 5 ug/ml. Immunofluorescence: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
Synonyms :
F2RL1, PAR2, Thrombin receptor-like 1, Protease-activated receptor 2, G-protein coupled receptor 11, GPR11, PAR-2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.