This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Prohibitin 2 / PHB2 Antibody (aa88-299) LS-B2316
catalog :
LS-B2316
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
82904
AntibodyName :
LS-B2316
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ Prohibitin 2 / PHB2 Antibody (aa88-299) LS-B2316
Specificity :
Recognizes mouse REA, Mr 37kD. Species cross-reactivity: human and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa88-299
PresentationDesc :
Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
His6-tagged fusion protein corresponding to aa88-299 of human REA-1. The immunizing sequence is identical in mouse.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
Prohibitin 2 / PHB2
StandardGeneSymbol :
PHB2
gene family :
Transcription factor
Reactivity :
Rat, Human
Usage :
ICC, IHC, IHC-P (5 µg/ml), IP, WB (0.5 - 2 µg/ml)
ShortWebDescription :
PHB2 antibody LS-B2316 is an unconjugated rabbit polyclonal antibody to PHB2 (Prohibitin 2) (aa88-299) from human. It is reactive with human and rat. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2316 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2316 was determined to be 5 ug/ml. Immunocytochemistry. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates REA from MCF-7 and 3T3 whole cell extracts. Immunocytochemistry: Nuclear staining of REA in paraformaldehyde fixed, Triton X-100 permeabilized MCF-7 cells.
Synonyms :
PHB2, BCAP37, BAP, D-prohibitin, p22, Prohibitin-2, B-cell associated protein, Bap37, PNAS-141, Prohibitin 2, REA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.