This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TRIM1 / MID2 Antibody (aa723-735) LS-B2277
catalog :
LS-B2277
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
82865
AntibodyName :
LS-B2277
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ TRIM1 / MID2 Antibody (aa723-735) LS-B2277
Specificity :
Human MID2. This antibody is expected to recognise both human isoforms of this protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa723-735
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence QESPYVSGMKTCH, from the C-Terminus of protein sequence according to NP_036348.2NP_438112.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
TRIM1 / MID2
BlockingPeptide :
LS-E25491
StandardGeneSymbol :
MID2
Reactivity :
Mouse, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.1 - 0.3 µg/ml)
ShortWebDescription :
MID2 antibody LS-B2277 is an unconjugated goat polyclonal antibody to MID2 (TRIM1) (aa723-735) from human. It is reactive with human, mouse and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2277 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2277 was determined to be 5 ug/ml.
Synonyms :
MID2, FXY2, Midin-2, Midline-2, Midin 2, Midline 2, Midline defect 2, RNF60, RING finger protein 60, TRIM1, Tripartite motif protein 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.