IHC-plus™ TLR4 Antibody (clone 1E11) LS-B2254 | LifeSpan Biosciences LS-B2254 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ TLR4 Antibody (clone 1E11) LS-B2254

catalog :

LS-B2254

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E11

reactivity :

human

application :

ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human	Elewa R, Abdallah M, Zouboulis C. Age-associated skin changes in innate immunity markers reflect a complex interaction between aging mechanisms in the sebaceous gland. J Dermatol. 2015;42:467-76 pubmed publisher

image

image 1 :

Anti-TLR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

82708

AntibodyName :

LS-B2254

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ TLR4 Antibody (clone 1E11) LS-B2254

Specificity :

Recognizes human Toll-like Receptor 4 (TLR4).

ClonalityDesc :

Monoclonal

CloneName :

1E11

AntibodyModification :

Unconjugated

PresentationDesc :

Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

ImmunogenType :

Recombinant protein

ImmunogenDesc :

Recombinant protein corresponding to amino acids 238-609 of human Toll-like Receptor 4 expressed in E. coli.

PurificationDesc :

Purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG2a

Gene :

TLR4

StandardGeneSymbol :

TLR4

gene family :

Toll-like Receptor

Reactivity :

Human

Usage :

ELISA (1:2000 - 1:4000), Flo, IHC, IHC-P (5 µg/ml)

ShortWebDescription :

TLR4 antibody LS-B2254 is an unconjugated mouse monoclonal antibody to human TLR4. Validated for ELISA, Flow and IHC. Tested on 20 paraffin-embedded human tissues.

UsageText :

Immunohistochemistry: LS-B2254 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2254 was determined to be 5 ug/ml.Flow Cytometry: 3ug/10e6 cellsOptimal dilutions to be determined by researcher.

Synonyms :

TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.