product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ EMA / MUC1 Antibody (FITC) LS-B2244
catalog :
LS-B2244
clonality :
monoclonal
host :
mouse
conjugate :
FITC
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Punnanitinont A, Kannisto E, Matsuzaki J, Odunsi K, Yendamuri S, Singh A, et al. Sublethal Radiation Affects Antigen Processing and Presentation Genes to Enhance Immunogenicity of Cancer Cells. Int J Mol Sci. 2020;21: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2244 image 1
Anti-MUC1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
82698
AntibodyName :
LS-B2244
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ EMA / MUC1 Antibody (FITC) LS-B2244
Specificity :
Recognizes human Mucin 1. Recognizes the peptide epitope Arg-Pro-Ala-Pro within the protein core of the mucin.
ClonalityDesc :
Monoclonal
AntibodyModification :
FITC
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
ImmunogenDesc :
Urinary MUC-1 mucin.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C. Do not freeze. Protect from light.
IsotypeName :
IgG3
Gene :
EMA / MUC1
StandardGeneSymbol :
MUC1
gene family :
Mucin
Reactivity :
Human
Usage :
Flo, IF, IHC, IHC-P (1:50)
ShortWebDescription :
MUC1 antibody LS-B2244 is an FITC-conjugated mouse monoclonal antibody to human MUC1 (EMA). Validated for Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2244 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2244 was determined to be 1:50. Flow Cytometry: Neat-1:10. Use 10 ul to label 10^6 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.