This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ LAG3 Antibody (aa70-99, clone 17B4, FITC) LS-B2237
catalog :
LS-B2237
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
17B4
reactivity :
macaque, human
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 4
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Hoffmann M, Pantazis N, Martin G, Hickling S, Hurst J, Meyerowitz J, et al. Exhaustion of Activated CD8 T Cells Predicts Disease Progression in Primary HIV-1 Infection. PLoS Pathog. 2016;12:e1005661 pubmed publisher
  • flow cytometry; human
Hurst J, Hoffmann M, Pace M, Williams J, Thornhill J, Hamlyn E, et al. Immunological biomarkers predict HIV-1 viral rebound after treatment interruption. Nat Commun. 2015;6:8495 pubmed publisher
  • immunohistochemistry; macaque
Phillips B, Mehra S, Ahsan M, Selman M, Khader S, Kaushal D. LAG3 expression in active Mycobacterium tuberculosis infections. Am J Pathol. 2015;185:820-33 pubmed publisher
Kroy D, Ciuffreda D, Cooperrider J, Tomlinson M, Hauck G, Aneja J, et al. Liver environment and HCV replication affect human T-cell phenotype and expression of inhibitory receptors. Gastroenterology. 2014;146:550-61 pubmed publisher
product information
AntibodyID :
80568
AntibodyName :
LS-B2237
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ LAG3 Antibody (aa70-99, clone 17B4, FITC) LS-B2237
Specificity :
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
ClonalityDesc :
Monoclonal
CloneName :
17B4
AntibodyModification :
FITC
AntigenModification :
aa70-99
PresentationDesc :
PBS containing 50mM Tris-HCl, 1% BSA, 0.02% sodium azide.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-Terminus D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%).
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 4°C. Protect from light.
IsotypeName :
IgG1
Gene :
LAG3
StandardGeneSymbol :
LAG3
Reactivity :
Human
Usage :
Flo (1:100), IHC, IHC-P (10 µg/ml)
ShortWebDescription :
LAG3 antibody LS-B2237 is an FITC-conjugated mouse monoclonal antibody to human LAG3 (aa70-99). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
UsageText :
Immunohistochemistry: LS-B2237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2237 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
LAG3, CD223 antigen, LAG-3, Protein FDC, CD223, FDC, Lymphocyte-activation gene 3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.