This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Endostatin Antibody LS-B2229
catalog :
LS-B2229
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1837.46

The same clone is also sold as:
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
63911
AntibodyName :
LS-B2229
TargetSpecies :
Mouse
Host Species :
Mouse
Product Name :
IHC-plus™ Endostatin Antibody LS-B2229
Specificity :
Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
ClonalityDesc :
Monoclonal
CloneName :
1837.46
AntibodyModification :
Unconjugated
PresentationDesc :
50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Full-length recombinant murine endostatin
PurificationDesc :
Thiophilic adsorption
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
Endostatin
StandardGeneSymbol :
COL18A1
gene family :
Collagen
Reactivity :
Mouse, Rat, Human
Usage :
IHC, IHC-P (1:100), WB (1:100 - 1:500)
ShortWebDescription :
Endostatin antibody LS-B2229 is an unconjugated mouse monoclonal antibody to Endostatin from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.
UsageText :
Immunohistochemistry: LS-B2229 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2229 was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Synonyms :
COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.