product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ OCLN / Occludin Antibody (C-Terminus) LS-B2187
catalog :
LS-B2187
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Kumar R, Gong H, Liu L, Ramos Solis N, Seye C, Derbigny W. TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection. PLoS ONE. 2019;14:e0207422 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2187 image 1
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
62441
AntibodyName :
LS-B2187
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ OCLN / Occludin Antibody (C-Terminus) LS-B2187
Specificity :
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
A GST fusion protein consisting of the C-Terminus 150 amino acids of human occludin fused to GST.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
OCLN / Occludin
StandardGeneSymbol :
OCLN
Reactivity :
Mouse, Dog, Rat, Human
Usage :
ELISA (0.1 - 1 µg/ml), IF, IHC-P (2.5 µg/ml), IP, WB (1 - 3 µg/ml)
ShortWebDescription :
Occludin antibody LS-B2187 is an unconjugated rabbit polyclonal antibody to Occludin (OCLN) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.
Synonyms :
OCLN, Occludin, BLCPMG
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.