This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CLDN12 / Claudin 12 Antibody (N-Terminus) LS-B2181
catalog :
LS-B2181
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
62435
AntibodyName :
LS-B2181
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ CLDN12 / Claudin 12 Antibody (N-Terminus) LS-B2181
Specificity :
Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide derived from the N-Terminus of mouse and human Claudin 12
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
CLDN12 / Claudin 12
StandardGeneSymbol :
CLDN12
gene family :
Claudin
Reactivity :
Mouse, Human
Usage :
IF (5 µg/ml), IHC, IHC-P (5 µg/ml), WB (1 - 3 µg/ml)
ShortWebDescription :
Claudin 12 antibody LS-B2181 is an unconjugated rabbit polyclonal antibody to Claudin 12 (CLDN12) (N-Terminus) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2181 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2181 was determined to be 5 ug/ml. Immunofluorescence: 5 ug/ml, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.
Synonyms :
CLDN12, Claudin 12, Claudin-12
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.