IHC-plus™ ABCB11 / BSEP Antibody (aa705-718) LS-B2170 | LifeSpan Biosciences LS-B2170 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ ABCB11 / BSEP Antibody (aa705-718) LS-B2170

catalog :

LS-B2170

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 1a	El Guindi M, Sira M, Hussein M, Ehsan N, Elsheikh N. Â Hepatic immunohistochemistry of bile transporters in progressive familial intrahepatic cholestasis. Ann Hepatol. 2016;15:222-9 pubmed publisher

image

image 1 :

Anti-ABCB11 / BSEP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

WB staining of crude plasma membrane purified from human liver tissue collected by fine needle biopsy.

product information

AntibodyID :

62424

AntibodyName :

LS-B2170

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ ABCB11 / BSEP Antibody (aa705-718) LS-B2170

Specificity :

Recognizes human BSEP. Does not cross-react with human P-glycoprotein.

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa705-718

PresentationDesc :

PBS pH 7.2, 0.7% BSA, 100mM sodium azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%); Marmoset, Dog (93%).

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG1

Gene :

ABCB11 / BSEP

StandardGeneSymbol :

ABCB11

gene family :

Transporter

Subfamily :

ATP-binding cassette - ABCB/MDR

Reactivity :

Human

Usage :

ICC, IHC, IHC-P (5 µg/ml), WB

ShortWebDescription :

BSEP antibody LS-B2170 is an unconjugated mouse monoclonal antibody to human BSEP (ABCB11) (aa705-718). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.

UsageText :

Immunohistochemistry: LS-B2170 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2170 was determined to be 5 ug/ml.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.

Synonyms :

ABCB11, ABC16, Bile salt export pump, BSEP, PGY4, Sister p-glycoprotein, BRIC2, PFIC-2, PFIC2, SPGP

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.