product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SIPR3 / EDG3 / S1P3 Antibody (aa12-25) LS-B2155
catalog :
LS-B2155
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Gril B, Paranjape A, Woditschka S, Hua E, Dolan E, Hanson J, et al. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. Nat Commun. 2018;9:2705 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2155 image 1
Anti-EDG3 antibody IHC of human prostate, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
62409
AntibodyName :
LS-B2155
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SIPR3 / EDG3 / S1P3 Antibody (aa12-25) LS-B2155
Specificity :
Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa12-25
PresentationDesc :
TBS, pH 7.4, 5 mg/ml BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (93%); Marmoset (86%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
SIPR3 / EDG3 / S1P3
StandardGeneSymbol :
S1PR3
gene family :
GPCR
Subfamily :
Lysophospholipid/Lysosphingolipid
Reactivity :
Human
Usage :
ICC, IHC, IHC-P (10 µg/ml), WB (3 µg/ml)
ShortWebDescription :
S1P3 antibody LS-B2155 is an unconjugated rabbit polyclonal antibody to human S1P3 (SIPR3 / EDG3) (aa12-25). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B2155 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2155 was determined to be 10 ug/ml.
Synonyms :
S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.