product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SERPINH1 / HSP47 Antibody LS-B2151
catalog :
LS-B2151
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; human
Cheng C, Tu W, Chen C, Chan H, Chen C, Chen H, et al. Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens. Sci Rep. 2018;8:1320 pubmed publisher
Cheng C, Tu W, Chen C, Chan H, Chen C, Chen H, et al. Proteomic Analysis of Thermal Regulation of Small Yellow Follicles in Broiler-Type Taiwan Country Chickens. J Poult Sci. 2018;55:120-136 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2151 image 1
Anti-SERPINH1 / HSP47 antibody IHC of human skin, fibroblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
62405
AntibodyName :
LS-B2151
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SERPINH1 / HSP47 Antibody LS-B2151
Specificity :
The antibody recognizes 47 kD Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.01% Thimerosal, 30% Glycerol, 0.5% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide surrounding amino acid 407 of human HSP47.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: -20°C; Long term: -70°C; Avoid freeze-thaw cycles.
Gene :
SERPINH1 / HSP47
BlockingPeptide :
LS-E48848
StandardGeneSymbol :
SERPINH1
Subfamily :
Serpin
Reactivity :
Chicken, Mouse, Rat, Human
Usage :
IHC, IHC-Fr (10 - 20 µg/ml), IHC-P (2.5 µg/ml), IP (10 - 20 µg/ml), WB (0.5 - 4 µg/ml)
ShortWebDescription :
HSP47 antibody LS-B2151 is an unconjugated rabbit polyclonal antibody to HSP47 (SERPINH1) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B2151 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2151 was determined to be 2.5 ug/ml.
Synonyms :
SERPINH1, 47 kDa heat shock protein, AsTP3, Colligin, Colligin-1, Collagen-binding protein, Colligin-2, CBP1, HSP47, OI10, Serpin H1, SERPINH2, CBP2, Collagen-binding protein 1, Colligen, gp46, PIG14, PPROM, RA-A47
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.