This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ ERBB2 / HER2 Antibody (aa1243-1255) LS-B2133
catalog :
LS-B2133
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
62386
AntibodyName :
LS-B2133
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ ERBB2 / HER2 Antibody (aa1243-1255) LS-B2133
Specificity :
Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1243-1255
PresentationDesc :
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
ImmunogenDesc :
A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%); Elephant, Bat, Bovine (92%); Opossum (85%).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
ERBB2 / HER2
StandardGeneSymbol :
ERBB2
gene family :
Protein Kinase
Subfamily :
EGF Receptor
Reactivity :
Mouse, Dog, Rat, Hamster, Pig, Horse, Human, Monkey
Usage :
IHC, IHC-Fr, IHC-P (5 - 10 µg/ml), IP, WB (0.5 - 2 µg/ml)
ShortWebDescription :
HER2 antibody LS-B2133 is an unconjugated rabbit polyclonal antibody to HER2 (ERBB2) (aa1243-1255) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
UsageText :
Immunohistochemistry: LS-B2133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2133 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 ug immunoprecipitated c-erbB-2 from 500 ug of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Synonyms :
ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.