antibody

PathPlus™ SAA1 / SAA / Serum Amyloid A Antibody LS-B2130

LS-B2130

monoclonal

mouse

nonconjugated

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

LS-B2130

Human

Mouse

PathPlus™ SAA1 / SAA / Serum Amyloid A Antibody LS-B2130

Recognizes natural and recombinant human SAA. Does not show any cross-reactivity with other human cytokines or growth factors tested such as IL-1b, IL-8, MCAF, TGF-b and EGF.

Monoclonal

Unconjugated

PBS, pH 7.0

Recombinant protein

Highly purified recombinant human SAA, 12kD

Protein G purified

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG1,k

SAA1 / SAA / Serum Amyloid A

SAA1

Human

ELISA (0.3 µg/ml), IHC, IHC-P (5 µg/ml), SwELISA, WB

Serum Amyloid A antibody LS-B2130 is an unconjugated mouse monoclonal antibody to human Serum Amyloid A (SAA1 / SAA). Validated for ELISA, IHC, SwELISA and WB. Cited in 1 publication.

Immunohistochemistry: LS-B2130 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2130 was determined to be 5 ug/ml. ELISA (capture coating): 0.3 ug/ml Detects 1.1 ng/ml of SAA in serum/plasma or 0.6 ng/ml of SAA in medium with an assay range of 0 to 80 ng/ml. Suggested substrate: TMB. Western Blot: 0.1-0.5 ug allows visualization of 100 ng/lane of recombinant human SAA. Used in sandwich ELISA as the capture antibody with LS-B2208 as the detection antibody.

SAA1, Serum amyloid A protein, Serum Amyloid A, Serum amyloid A-1 protein, TP53I4, SAA, Serum amyloid A1, PIG4

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