This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TJP1 / ZO-1 Antibody (aa463-1109) LS-B2129
catalog :
LS-B2129
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
62382
AntibodyName :
LS-B2129
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ TJP1 / ZO-1 Antibody (aa463-1109) LS-B2129
Specificity :
Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa463-1109
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Fusion protein
ImmunogenDesc :
A 69 kDa fusion protein corresponding to aa 463-1109 of human ZO-1 cDNA. This sequence lies N-Terminus to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
TJP1 / ZO-1
StandardGeneSymbol :
TJP1
gene family :
Occludin
Reactivity :
Mouse, Dog, Guinea pig, Rat, Human
Usage :
ELISA, IHC, IHC-Fr, IHC-P (5 µg/ml), IP, WB (0.5 - 2 µg/ml)
ShortWebDescription :
ZO-1 antibody LS-B2129 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) (aa463-1109) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2129 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Immunoprecipitation: 2-5 ug/reaction.
Synonyms :
TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.