product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TNF Alpha Antibody (clone 52B83) LS-B2123
catalog :
LS-B2123
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
52B83
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; fig 3
Cantley M, Dharmapatni A, Algate K, Crotti T, Bartold P, Haynes D. Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue. J Periodontal Res. 2016;51:143-51 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2123 image 1
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
62375
AntibodyName :
LS-B2123
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ TNF Alpha Antibody (clone 52B83) LS-B2123
Specificity :
Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
ClonalityDesc :
Monoclonal
CloneName :
52B83
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenDesc :
Human TNFa.
PurificationDesc :
Purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
TNF Alpha
StandardGeneSymbol :
TNF
gene family :
TNF
Reactivity :
Mouse, Guinea pig, Human, Monkey
Usage :
ELISA, Flo, IHC, IHC-Fr, IHC-P (2.5 µg/ml), WB (1:5)
ShortWebDescription :
TNF Alpha antibody LS-B2123 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. It is reactive with human, mouse, guinea pig and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2123 was determined to be 2.5 ug/ml. Non-specific background staining is observed in connective tissues.
Synonyms :
TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.