IHC-plus™ VTN / Vitronectin Antibody LS-B2118 | LifeSpan Biosciences LS-B2118 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ VTN / Vitronectin Antibody LS-B2118

catalog :

LS-B2118

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 8h	Merritt N, Garcia K, Rajendran D, Lin Z, Zhang X, Mitchell K, et al. TAZ-CAMTA1 and YAP-TFE3 alter the TAZ/YAP transcriptome by recruiting the ATAC histone acetyltransferase complex. elife. 2021;10: pubmed publisher

image

image 1 :

Anti-Vitronectin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

62370

AntibodyName :

LS-B2118

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ VTN / Vitronectin Antibody LS-B2118

Specificity :

Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

PresentationDesc :

PBS, pH 7.4, 15 mM Sodium Azide

ImmunogenType :

Purified protein

ImmunogenDesc :

Human vitronectin purified from plasma by heparin-affinity chromatography.

PurificationDesc :

Affinity purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG1,k

Gene :

VTN / Vitronectin

StandardGeneSymbol :

VTN

gene family :

Matrix Protein

Reactivity :

Goat, Dog, Bovine, Cat, Horse, Human

Usage :

ELISA (1:30000), IHC, IHC-P (2.5 µg/ml), WB (1:50)

ShortWebDescription :

Vitronectin antibody LS-B2118 is an unconjugated mouse monoclonal antibody to Vitronectin (VTN) from human. It is reactive with human, bovine, cat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

UsageText :

Immunohistochemistry: LS-B2118 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2118 was determined to be 2.5 ug/ml. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC.

Synonyms :

VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.