antibody

IHC-plus™ Collagen I Antibody (Biotin) LS-B209

LS-B209

polyclonal

domestic rabbit

biotin

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

LS-B209

Mammal

Rabbit

IHC-plus™ Collagen I Antibody (Biotin) LS-B209

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Polyclonal

Biotin

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Purified protein

Collagen Type I from human and bovine placenta.

Affinity chromatography

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Reconstitute in 100 µL of deionized water.

IgG

Collagen I

Mouse, Bovine, Rat, Mammal, Human

ELISA (1:3000 - 1:6000), Flo, IHC, IHC-P (10 µg/ml), IP (1:100), WB (1:3000 - 1:6000)

Collagen I antibody LS-B209 is a biotin-conjugated rabbit polyclonal antibody to Collagen I from mammal. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC, IP and WB.

Immunohistochemistry: LS-B209 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B209 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Worldwide