This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TP73 / p73 Antibody (N-Terminus, clone 5B429) LS-B2086
catalog :
LS-B2086
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5B429

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
53036
AntibodyName :
LS-B2086
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ TP73 / p73 Antibody (N-Terminus, clone 5B429) LS-B2086
Specificity :
Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
ClonalityDesc :
Monoclonal
CloneName :
5B429
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenDesc :
Full-length human p73. It reacts with an epitope that is located on the N-Terminus of human p73. It does not cross react with p53.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
TP73 / p73
StandardGeneSymbol :
TP73
Reactivity :
Mouse, Human
Usage :
ICC, IHC, IHC-P (5 µg/ml), IP, WB (2 µg/ml)
ShortWebDescription :
P73 antibody LS-B2086 is an unconjugated mouse monoclonal antibody to p73 (TP73) (N-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2086 was determined to be 5 ug/ml.
Synonyms :
TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.