This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SMAD6 Antibody (aa85-99) LS-B2065
catalog :
LS-B2065
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
53015
AntibodyName :
LS-B2065
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SMAD6 Antibody (aa85-99) LS-B2065
Specificity :
A 52 kD band is observed.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa85-99
PresentationDesc :
PBS, 0.05% Sodium Azide, 0.05% BSA
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%); Dog, Bovine, Opossum (93%); Gibbon, Marmoset, Rat (87%); Elephant (80%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
SMAD6
StandardGeneSymbol :
SMAD6
Reactivity :
Pig, Human
Usage :
IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)
ShortWebDescription :
SMAD6 antibody LS-B2065 is an unconjugated rabbit polyclonal antibody to SMAD6 (aa85-99) from human. It is reactive with human and pig. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B2065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2065 was determined to be 5 ug/ml.
Synonyms :
SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.