product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ OLFM4 / Olfactomedin 4 Antibody (aa400-450) LS-B2055
catalog :
LS-B2055
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
acetylated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Suzuki T, Yamazaki H, Honda K, Ryo E, Kaneko A, Ota Y, et al. Altered DNA methylation is associated with aberrant stemness gene expression in early‑stage HNSCC. Int J Oncol. 2019;55:915-924 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2055 image 1
Anti-OLFM4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
53005
AntibodyName :
LS-B2055
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ OLFM4 / Olfactomedin 4 Antibody (aa400-450) LS-B2055
Specificity :
A portion of amino acids 400-450 of human Olfactomedin 4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa400-450
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide corresponding to amino acids 400-419 (YVYNDGYLLNYDLSVLQKPQ) of human OLFM4 was used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (95%); Monkey, Mouse, Rat (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Avoid freeze-thaw cycles.
Gene :
OLFM4 / Olfactomedin 4
StandardGeneSymbol :
OLFM4
gene family :
Olfactomedin
Reactivity :
Human
Usage :
IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)
ShortWebDescription :
Olfactomedin 4 antibody LS-B2055 is an unconjugated rabbit polyclonal antibody to human Olfactomedin 4 (OLFM4) (aa400-450). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B2055 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2055 was determined to be 5 ug/ml.
Synonyms :
OLFM4, Antiapoptotic protein GW112, HOLfD, HGC-1, GW112, OlfD, OLM4, UNQ362, Olfactomedin 4, Olfactomedin-4, BA209J19.1, GC1, HCG-1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.