product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HSPD1 / HSP60 Antibody (aa150-200) LS-B2040
catalog :
LS-B2040
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 3g
Maiti P, Kim H, Tu Y, Barrientos A. Human GTPBP10 is required for mitoribosome maturation. Nucleic Acids Res. 2018;46:11423-11437 pubmed publisher
Timón Gómez A, Garlich J, Stuart R, Ugalde C, Barrientos A. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep. 2020;31:107607 pubmed publisher
Burstein S, Valsecchi F, Kawamata H, Bourens M, Zeng R, Zuberi A, et al. In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions. Hum Mol Genet. 2018;27:160-177 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B2040 image 1
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
52990
AntibodyName :
LS-B2040
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ HSPD1 / HSP60 Antibody (aa150-200) LS-B2040
Specificity :
The amino acid sequence used as immunogen is 100% homologous in a broad range of species including human, dog, mouse, rat, bovine, chimp, monkey, and orangutan.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa150-200
PresentationDesc :
PBS, 0.05% Sodium Azide, 0.2% Gelatin
ImmunogenType :
Protein fragment
ImmunogenDesc :
A portion of amino acids 150-200 of human Hsp60.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
HSPD1 / HSP60
StandardGeneSymbol :
HSPD1
Reactivity :
Dog, Bovine, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)
ShortWebDescription :
HSP60 antibody LS-B2040 is an unconjugated rabbit polyclonal antibody to HSP60 (HSPD1) (aa150-200) from human. It is reactive with human, bovine, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B2040 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2040 was determined to be 5 ug/ml.
Synonyms :
HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.