IHC-plus™ Histone H2B Antibody (aa111-125) LS-B2035 | LifeSpan Biosciences LS-B2035 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ Histone H2B Antibody (aa111-125) LS-B2035

catalog :

LS-B2035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; zebrafish ; 1:100; fig s1 western blot; zebrafish ; 1:500; fig s1	SpirÃ³ Z, Koh A, Tay S, See K, Winkler C. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep. 2016;6:27470 pubmed publisher

image

image 1 :

Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

52985

AntibodyName :

LS-B2035

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ Histone H2B Antibody (aa111-125) LS-B2035

Specificity :

A 15 kD band is observed. This is routinely quality controlled in human lysates.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa111-125

PresentationDesc :

PBS, 0.05% Sodium Azide, 0.05% BSA

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG

Gene :

Histone H2B

Reactivity :

Chicken, Zebrafish, Insect, Drosophila, Bovine, Mouse, Rat, Human

Usage :

IHC, IHC-P (5 µg/ml), WB (2 µg/ml)

ShortWebDescription :

Histone H2B antibody LS-B2035 is an unconjugated rabbit polyclonal antibody to Histone H2B (aa111-125) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B2035 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2035 was determined to be 5 ug/ml.

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.