product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CD177 Antibody (clone MEM-166) LS-B1953
catalog :
LS-B1953
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MEM-166
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Reference
Lu Y, Dong B, Xu F, Xu Y, Pan J, Song J, et al. CXCL1-LCN2 paracrine axis promotes progression of prostate cancer via the Src activation and epithelial-mesenchymal transition. Cell Commun Signal. 2019;17:118 pubmed publisher
Pradhan Nabzdyk L, Kuchibhotla S, Guthrie P, Chun M, Auster M, Nabzdyk C, et al. Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. J Vasc Surg. 2013;58:766-75.e12 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1953 image 1
Anti-CD177 antibody IHC of human intravascular neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1953 image 2
Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
product information
AntibodyID :
51242
AntibodyName :
LS-B1953
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CD177 Antibody (clone MEM-166) LS-B1953
Specificity :
Human granulocytes
ClonalityDesc :
Monoclonal
CloneName :
MEM-166
AntibodyModification :
Unconjugated
PresentationDesc :
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
ImmunogenType :
Cells
ImmunogenDesc :
Cells
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C. Store undiluted.
IsotypeName :
IgG1
Gene :
CD177
StandardGeneSymbol :
CD177
Reactivity :
Human, Monkey
Usage :
Flo (1 µg/10E6 cells), IHC, IHC-P (10 µg/ml), IP, WB
ShortWebDescription :
CD177 antibody LS-B1953 is an unconjugated mouse monoclonal antibody to CD177 from human. It is reactive with human and monkey. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B1953 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1953 was determined to be 10 ug/ml.
Synonyms :
CD177, CD177 molecule, CD177 antigen, Cell surface receptor, HNA-2a, HNA2A, NB1, PRV-1, Polycythemia rubra vera 1, PRV1, NB1 glycoprotein, NB1 GP
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.