product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ DKK1 Antibody (aa250-262) LS-B194
catalog :
LS-B194
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Tian L, Wen A, Dong S, Yan P. Molecular Characterization of Microtubule Affinity-Regulating Kinase4 from Sus scrofa and Promotion of Lipogenesis in Primary Porcine Placental Trophoblasts. Int J Mol Sci. 2019;20: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B194 image 1
Anti-DKK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B194 image 2
DKK1 antibody (1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
product information
AntibodyID :
28115
AntibodyName :
LS-B194
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ DKK1 Antibody (aa250-262) LS-B194
Specificity :
Human DKK1.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa250-262
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence CDHHQASNSSRLHT, from the C-Terminus of protein sequence according to NP_036374.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
DKK1
BlockingPeptide :
LS-E25759
StandardGeneSymbol :
DKK1
Reactivity :
Mouse, Goat, Dog, Bovine, Pig, Horse, Human, Monkey
Usage :
IHC, IHC-P (10 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
ShortWebDescription :
DKK1 antibody LS-B194 is an unconjugated goat polyclonal antibody to DKK1 (aa250-262) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B194 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B194 was determined to be 10 ug/ml.
Synonyms :
DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.