product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ VCP Antibody (clone 4G9) LS-B1912
catalog :
LS-B1912
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4G9
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Reference
Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Infect Immun. 2012;80:1803-14 pubmed publisher
Yi L, Donsante A, Kennerson M, Mercer J, Garbern J, Kaler S. Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy. Hum Mol Genet. 2012;21:1794-807 pubmed publisher
Shchedrina V, Everley R, Zhang Y, Gygi S, Hatfield D, Gladyshev V. Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis. J Biol Chem. 2011;286:42937-48 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1912 image 1
Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1912 image 2
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI
image 3 :
LifeSpan Biosciences LS-B1912 image 3
Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
product information
AntibodyID :
48321
AntibodyName :
LS-B1912
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ VCP Antibody (clone 4G9) LS-B1912
Specificity :
Recombinant human valosin containing protein
ClonalityDesc :
Monoclonal
CloneName :
4G9
AntibodyModification :
Unconjugated
PresentationDesc :
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human VCP.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C. Do not freeze.
IsotypeName :
IgG1
Gene :
VCP
StandardGeneSymbol :
VCP
Reactivity :
Human
Usage :
ICC, IF (1 - 4 µg/ml), IHC, IHC-P (5 µg/ml), IP, WB
ShortWebDescription :
VCP antibody LS-B1912 is an unconjugated mouse monoclonal antibody to human VCP. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B1912 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1912 was determined to be 5 ug/ml.
Synonyms :
VCP, 15S Mg(2+)-ATPase p97 subunit, ALS14, TER ATPase, TERA, Valosin containing protein, Yeast Cdc48p homolog, Valosin-containing protein, IBMPFD, p97
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.