product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ACKR3 / CXCR7 Antibody (aa100-150) LS-B1815
catalog :
LS-B1815
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, proximity ligation assay
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • proximity ligation assay; human; 1:400; fig 1
  • flow cytometry; human
  • proximity ligation assay; mouse
  • flow cytometry; mouse
Evans A, Tripathi A, Laporte H, Brueggemann L, Singh A, Albee L, et al. New Insights into Mechanisms and Functions of Chemokine (C-X-C Motif) Receptor 4 Heteromerization in Vascular Smooth Muscle. Int J Mol Sci. 2016;17: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1815 image 1
Anti-CXCR7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
48224
AntibodyName :
LS-B1815
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ACKR3 / CXCR7 Antibody (aa100-150) LS-B1815
Specificity :
A portion of amino acids 100-150 of human RDC1.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa100-150
PresentationDesc :
Tris-Glycine, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide (QHNQWPMGELTC) corresponding amino acids 106-117 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum (100%); Platypus, Xenopus (92%); Turkey (83%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
ACKR3 / CXCR7
StandardGeneSymbol :
ACKR3
gene family :
GPCR
Subfamily :
Chemokine
Reactivity :
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
Usage :
Flo (1 - 10 µg/ml), IHC, IHC-P (10 µg/ml), WB (4 - 6 µg/ml)
ShortWebDescription :
CXCR7 antibody LS-B1815 is an unconjugated rabbit polyclonal antibody to CXCR7 (ACKR3) (aa100-150) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B1815 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1815 was determined to be 10 ug/ml.
Synonyms :
ACKR3, Chemokine orphan receptor 1, CMKOR1, CXCR7, CXC-R7, GPRN1, RDC1, RDC-1, CXCR-7, G-protein coupled receptor 159, GPR159
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.