product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ IDO1 / IDO Antibody (aa129-141) LS-B1746
catalog :
LS-B1746
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • ELISA; human
Hu P, Hunt N, Arfuso F, Shaw L, Uddin M, Zhu M, et al. Increased Indoleamine 2,3-Dioxygenase and Quinolinic Acid Expression in Microglia and Müller Cells of Diabetic Human and Rodent Retina. Invest Ophthalmol Vis Sci. 2017;58:5043-5055 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1746 image 1
Anti-IDO1 / IDO antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1746 image 2
IDO1 / IDO antibody (0.5µg/ml) staining of Human Lymph Node (A), Spleen (B) and Placenta (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
product information
AntibodyID :
47581
AntibodyName :
LS-B1746
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ IDO1 / IDO Antibody (aa129-141) LS-B1746
Specificity :
Human IDO1 / IDO.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa129-141
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence CVLANWKKKDPNK, from the internal region of the protein sequence according to NP_002155.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
IDO1 / IDO
BlockingPeptide :
LS-E25961
StandardGeneSymbol :
IDO1
Reactivity :
Mouse, Human, Monkey
Usage :
IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1.5 µg/ml)
ShortWebDescription :
IDO antibody LS-B1746 is an unconjugated goat polyclonal antibody to IDO (IDO1) (aa129-141) from human. It is reactive with human, mouse and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B1746 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1746 was determined to be 2.5 ug/ml.
Synonyms :
IDO1, IDO, Indole 2,3-dioxygenase, Indolamine 2,3 dioxygenase, IDO-1, INDO, Indoleamine 2,3-dioxygenase, Indoleamine 2,3-dioxygenase 1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.