product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HRH1 / Histamine H1 Receptor Antibody (aa471-484) LS-B1745
catalog :
LS-B1745
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. JCI Insight. 2017;2:e86492 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1745 image 1
Anti-HRH1 / Histamine H1 Receptor antibody IHC of human small intestine, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
47580
AntibodyName :
LS-B1745
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ HRH1 / Histamine H1 Receptor Antibody (aa471-484) LS-B1745
Specificity :
Human HRH1 / Histamine H1 Receptor. Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa471-484
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence CNENFKKTFKRILH, from the C-Terminus of protein sequence according to NP_000852.1NP_001091681.1NP_001091682.1NP_001091683.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
HRH1 / Histamine H1 Receptor
BlockingPeptide :
LS-E25938
StandardGeneSymbol :
HRH1
gene family :
GPCR
Subfamily :
Histamine
Reactivity :
Bat, Human
Usage :
IF, IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000)
ShortWebDescription :
Histamine H1 Receptor antibody LS-B1745 is an unconjugated goat polyclonal antibody to Histamine H1 Receptor (HRH1) (aa471-484) from human. It is reactive with human and bat. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B1745 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1745 was determined to be 2.5 ug/ml.
Synonyms :
HRH1, H1 histamine receptor, H1 receptor, Histamine 1 receptor, Histamine H1 receptor, H1-R, H1R, Histamine H(1) receptor, Histamine receptor H1, HisH1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.