product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) LS-B1675
catalog :
LS-B1675
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
2A3
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Molli P, Li D, Bagheri Yarmand R, Pakala S, Katayama H, Sen S, et al. Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. J Cell Biol. 2010;190:101-14 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1675 image 1
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1675 image 2
product information
AntibodyID :
47456
AntibodyName :
LS-B1675
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) LS-B1675
Specificity :
Modified peptide
ClonalityDesc :
Monoclonal
CloneName :
2A3
AntibodyModification :
Unconjugated
AntigenModification :
pThr210
PresentationDesc :
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced from tissue culture supernatant.
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from human PLK1.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C. Avoid freezing.
IsotypeName :
IgG1
Gene :
PLK1 / PLK-1
StandardGeneSymbol :
PLK1
gene family :
Protein Kinase
Subfamily :
Polo
Reactivity :
Human
Usage :
IHC, IHC-P (15 µg/ml), WB
ShortWebDescription :
PLK-1 antibody LS-B1675 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1) (pThr210). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml.
Synonyms :
PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.