product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NFKB1 / NF-Kappa-B Antibody (clone 4D1) LS-B1668
catalog :
LS-B1668
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4D1
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 4
Reference
Lin D, Lavender H, Soilleux E, O Callaghan C. NF-?B regulates MICA gene transcription in endothelial cell through a genetically inhibitable control site. J Biol Chem. 2012;287:4299-310 pubmed publisher
Geldmeyer Hilt K, Heine G, Hartmann B, Baumgrass R, Radbruch A, Worm M. 1,25-dihydroxyvitamin D3 impairs NF-?B activation in human naïve B cells. Biochem Biophys Res Commun. 2011;407:699-702 pubmed publisher
Kao C, Kim C, Huang F, Wu R. Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium. J Biol Chem. 2008;283:15309-18 pubmed publisher
Huang F, Kao C, Wachi S, Thai P, Ryu J, Wu R. Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells. J Immunol. 2007;179:6504-13 pubmed
image
image 1 :
LifeSpan Biosciences LS-B1668 image 1
Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1668 image 2
MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-kappaB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
product information
AntibodyID :
47449
AntibodyName :
LS-B1668
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ NFKB1 / NF-Kappa-B Antibody (clone 4D1) LS-B1668
Specificity :
Recombinant full-length human NF-kB p50
ClonalityDesc :
Monoclonal
CloneName :
4D1
AntibodyModification :
Unconjugated
PresentationDesc :
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Recombinant human NFKB1.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at 4°C. Avoid freezing.
IsotypeName :
IgG1
Gene :
NFKB1 / NF-Kappa-B
StandardGeneSymbol :
NFKB1
gene family :
Apoptosis
Reactivity :
Human
Usage :
IF, IHC, IHC-P (15 µg/ml), WB
ShortWebDescription :
NF-Kappa-B antibody LS-B1668 is an unconjugated mouse monoclonal antibody to human NF-Kappa-B (NFKB1). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
UsageText :
Immunohistochemistry: LS-B1668 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1668 was determined to be 15 ug/ml.
Synonyms :
NFKB1, Ebp- 1, EBP-1, NF-KappaB p50, NFkappaB, NFKB-p50, KBF1, NF-kappa-B, NF-kappaB, NF-kB1, NFKB-p105, p105, p50, DNA binding factor KBF1, DNA-binding factor KBF1, NF-kappabeta
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.