This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ EGLN1 / PHD2 Antibody (aa1-50) LS-B166
catalog :
LS-B166
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
28087
AntibodyName :
LS-B166
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ EGLN1 / PHD2 Antibody (aa1-50) LS-B166
Specificity :
Human HIF prolyl hydroxylase 2. There is 83% homology between the immunogen used for this and the rat and mouse proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1-50
PresentationDesc :
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
ImmunogenDesc :
The epitope recognized by this antibody maps to a region between residues 1 and 50 of human Prolyl Hydroxylase Domain-Containing Protein 2 using the numbering given in entry NP_071334.1 (GeneID 54583)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: 4°C; Long term: -70°C.
Gene :
EGLN1 / PHD2
StandardGeneSymbol :
EGLN1
Reactivity :
Rat, Human
NonReactiveSpecies :
Mouse
Usage :
Flo (1:10 - 1:1000), ICC, IHC, IHC-P (10 µg/ml), IP, WB (1:500 - 1:2500)
ShortWebDescription :
PHD2 antibody LS-B166 is an unconjugated rabbit polyclonal antibody to PHD2 (EGLN1) (aa1-50) from human. It is reactive with human and rat. Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B166 was determined to be 10 ug/ml.
Synonyms :
EGLN1, C1orf12, EGL9, ECYT3, Egl nine-like protein 1, HIFPH2, HIF prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, ZMYND6, PHD2, SM20, EGL nine (C.elegans) homolog 1, Egl nine homolog 1, HPH-2, HPH2, SM-20
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.