IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus) LS-B1625 | LifeSpan Biosciences LS-B1625 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus) LS-B1625

catalog :

LS-B1625

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 7

Reference
Zhao C, Zhang J, Ma L, Wu H, Zhang H, Su J, et al. GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/β-Catenin Signaling Pathway. Onco Targets Ther. 2020;13:6265-6277 pubmed publisher
Li Q, Zhou X, Fang Z, Zhou H. Knockdown of KLK12 inhibits viability and induces apoptosis in human colorectal cancer HT-29 cell line. Int J Mol Med. 2019;44:1667-1676 pubmed publisher
Zhu L, Yang S, Wang J. miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1. Int J Mol Med. 2019;44:1824-1832 pubmed publisher
Li G, Jiang X, Liu L, Liu X, Liu H, Zhang Z. Effect of estradiol on high glucose‑induced osteoblast injury. Mol Med Rep. 2019;20:3019-3026 pubmed publisher
Zhang Y, Yang J, Zhou X, Wang N, Li Z, Zhou Y, et al. Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells. Int J Mol Med. 2019;44:1357-1365 pubmed publisher
Sun C, Zhang G, Cheng S, Qian H, Li D, Liu M. URG11 promotes proliferation and induced apoptosis of LNCaP cells. Int J Mol Med. 2019;43:2075-2085 pubmed publisher
Sun M, Liu C, Zhao N, Meng K, Zhang Z. Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. Exp Ther Med. 2018;15:5215-5220 pubmed publisher

image

image 1 :

Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

image 2 :

Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.

image 3 :

Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.

product information

AntibodyID :

47398

AntibodyName :

LS-B1625

TargetSpecies :

Mouse

Host Species :

Rabbit

Product Name :

IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus) LS-B1625

Specificity :

Peptide-KLH, NH2 terminus

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

N-Terminus

PresentationDesc :

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

Synthetic peptide from mouse ACTB / Beta-actin.

PurificationDesc :

Affinity purified

RecommendedStorageDesc :

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG

Gene :

ACTB / Beta Actin

StandardGeneSymbol :

ACTB

Reactivity :

Mouse, Rat, Human

Usage :

ICC, IHC, IHC-P (1:100), WB

ShortWebDescription :

Beta Actin antibody LS-B1625 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 17 publications.

UsageText :

Immunohistochemistry: LS-B1625 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1625 was determined to be 1:100.

Synonyms :

ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage