product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PEA3 / ETV4 Antibody (aa181-230) LS-B1527
catalog :
LS-B1527
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; mouse
  • western blot; human
Okimoto R, Breitenbuecher F, Olivas V, Wu W, Gini B, Hofree M, et al. Inactivation of Capicua drives cancer metastasis. Nat Genet. 2017;49:87-96 pubmed publisher
Lu Y, Ju H, Liu Z, Chen D, Wang Y, Zhao Q, et al. ME1 Regulates NADPH Homeostasis to Promote Gastric Cancer Growth and Metastasis. Cancer Res. 2018;78:1972-1985 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1527 image 1
Anti-ETV4 / PEA3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
45462
AntibodyName :
LS-B1527
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ PEA3 / ETV4 Antibody (aa181-230) LS-B1527
Specificity :
Human ETV4 / PEA3
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa181-230
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa181-230 of human ETV4 (P43268, NP_001977). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Pig (100%); Marmoset, Bat, Rabbit, Horse (92%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
PEA3 / ETV4
BlockingPeptide :
LS-E11278
StandardGeneSymbol :
ETV4
Reactivity :
Mouse, Bovine, Rat, Pig, Human
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
ETV4 antibody LS-B1527 is an unconjugated rabbit polyclonal antibody to ETV4 (PEA3) (aa181-230) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B1527 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1527 was determined to be 5 ug/ml.
Synonyms :
ETV4, ETS translocation variant 4, E1A-F, E1AF, Ets variant 4, PEA3, Protein PEA3, PEAS3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.