This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ALOX5 / 5-LOX Antibody (aa130-149) LS-B1499
catalog :
LS-B1499
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
45434
AntibodyName :
LS-B1499
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ALOX5 / 5-LOX Antibody (aa130-149) LS-B1499
Specificity :
Human and rat amino acids 130-149 (QHRRKELETRQKQYRWMEWN); The sequence is 95% homologous to 5-LO from mouse and hamster
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa130-149
PresentationDesc :
TBS, pH 7.4
ImmunogenDesc :
Human and rat amino acids 130-149 (QHRRKELETRQKQYRWMEWN). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Bovine, Elephant, Panda (100%); Monkey, Mouse, Hamster, Rabbit, Opossum (95%); Bat (90%); Turkey, Chicken, Salmon, Zebrafish (80%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
ResuspensionLiquidDesc :
500 ul sterile ddH20
IsotypeName :
IgG
Gene :
ALOX5 / 5-LOX
StandardGeneSymbol :
ALOX5
gene family :
Lipoxygenase
Reactivity :
Bovine, Rat, Human
Usage :
ICC, IHC, IHC-P (1:50 - 1:100), WB
ShortWebDescription :
5-LOX antibody LS-B1499 is an unconjugated rabbit polyclonal antibody to 5-LOX (ALOX5) (aa130-149) from human. It is reactive with human, rat and bovine. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B1499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1499 was determined to be 10 ug/ml.
Synonyms :
ALOX5, 5-lipoxygenase, 5LPG, 5-LO, 5-LOX, Arachidonate 5-lipoxygenase, LOG5, LOX-5, Leukotriene A4 synthase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.